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The percentage of depolarization (corresponding to the proportion of viable 7AAD negative cells that were negative for DiIC(5)) is shown (P on D3 for shCont versus shAK2, n (e) Cell proliferation was analyzed following incorporation of EdU 3 and then 7 days after the initiation of T cell differentiation (P and P on D3 and D7, respectively, n (f) Flow cytometry analysis of GFP cells 28 days after the initiation of T cell differentiation in the shCont and shAK2 conditions.
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